Figure 2. Origins of the axial tissues in tail regeneration. A–D: Schematic representation of lineage labeling by tissue grafts expressing constitutive green fluorescent protein (GFP). A: GFP-positive neural plate grafted at st. 13, will constitutively label a section of the ventral spinal cord in the tadpole (see A′ and inset). After amputation, the entire ventral spinal cord regenerate will be GFP-positive (A′). B: GFP-positive notochord grafted at st. 13 will give rise to a GFP-positive section in the tadpole (B′). After amputation, the complete notochord in the regenerate is GFP positive (B′). C: GFP positive presomitic mesoderm grafted at st. 13 does not contain any muscle satellite cells, but labels muscle fibers in a section in the tadpole tail (C′). After amputation, no GFP-positive cells are found in the regenerate (C′). D: GFP-positive presomitic mesoderm grafted at st. 17 contains muscle satellite cells and labels similar to the early muscle grafts muscle fibers and satellite cells in a section of the tadpole tail (D′). After amputation, GFP-positive cells are seen in the regeneration bud and later in the newly formed muscle fibers of the regenerate (D′).
Image published in: Beck CW et al. (2009)
Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons.
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