Figure 3. Regeneration of Xenopus hindlimbs declines with age. A–C: Developing limbs at stages indicated. D–F: Whole-mounts of 5 day blastemas amputated at indicated stages through the ankle. G–I: Longitudinal sections of 5 day blastemas stained with hematoxylin and eosin, amputated at indicated stages through the ankle. The 5 days of regeneration adds at least one developmental stage; therefore, proximal limb histology corresponds to st. 53 (G), st. 55 (H), and st. 58 (I). Note that 5 day blastemas in younger stages are larger than in older stages (compare G–I). G′–I′: Higher magnification of distal blastema from pictures above. Note the prominent distal epidermis forming the apical epithelial cap (AEC; aec), while columnar basal epithelial cells (be) are lining the blastema. In st. 57 regenerates, no AEC forms and the wound is just covered by a simple wound epithelium (e). Black arrowheads indicate the amputation plane. fe, cartilage condensation for femur; at st. 57 the femur is partially ossified, t/f, cartilage condensation for tibia and fibula, at st. 57 tibia and fibula also contain bone; m, muscle; b, blastema. Scale bars = 250 μm.
Image published in: Beck CW et al. (2009)
Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons.
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