Fig. 1. A −6Kb:hhex:gfp transgene recapitulates hhex expression. (A) Diagram of the Xenopus laevis hhex genomic locus and −6.0Kb:hhex:gfp transgene. (B) The transgene recapitulates hhex expression in the anterior endoderm from blastula (st9.5) to stages 25. Transgenic embryos assayed by in situ hybridization for hhex or gfp mRNA and for GFP fluorescence with a confocal (st9.5 and 10) or stereomicroscope (st18–35). Embryos from st9.5 to 18 are bisected; in st9.5–11, dorsal is left; and in st18–35, anterior is right. The GFP image at st35 shows both a transgenic (top) and non-transgenic embryos (bottom). Two independent transgenic lines exhibited identical expression patterns. Arrowheads indicate endothelial cells (black), thyroid (white), and liver bud (yellow).
Image published in: Rankin SA et al. (2011)
Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| hhex.L | hex, tHex, XHex | X. laevis | Throughout NF stage 9 | endoderm anterior |
| hhex.L | hex, tHex, XHex | X. laevis | Throughout NF stage 10 | endoderm endomesoderm anterior |
| hhex.L | hex, tHex, XHex | X. laevis | Throughout NF stage 18 | endoderm foregut liver primordium liver diverticulum |
| hhex.L | hex, tHex, XHex | X. laevis | Throughout NF stage 25 | endoderm liver primordium endothelial cell vascular endothelium foregut alimentary system |
| hhex.L | hex, tHex, XHex | X. laevis | Throughout NF stage 35 and 36 | foregut liver primordium thyroid primordium posterior cardinal vein liver alimentary system |
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