Fig. S10. Geminin repression of mesodermal gene expression is not sensitive to inhibition of histone deacetylase activity. Embryos were injected with Gemininδcoil mRNA (300 pg; one cell out of two cells) and half of the embryos were treated from the two-cell stage (immediately after injection) with 100 nM TSA, followed by in situ hybridization at stage 11. (A,B) Geminin repressed Brachyury expression in untreated and TSA (100 nM)-treated embryos. (C) Fraction of embryos showing the phenotypes in A and B is indicated (additive quantitation from two experiments). (D) Gemininδcoil-injected embryos were used to prepare animal cap explants and were treated with Activin as before, plus and minus TSA co-treatment. Geminin repressed Activin-induced Brachyury expression with similar efficiency in the presence and absence of TSA treatment.
Image published in: Lim JW et al. (2011)
Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Permanent Image Page
Printer Friendly View
XB-IMG-49620