Fig. 4. Flag-BrunoL1 mRNA rescues the morpholino knockdown phenotype. (A) Control NF42 whole gut stained for elastase mRNA showing expression in the pancreas. (B) Whole gut from embryo injected with 15 ng of brunol1 morpholino. Elastase expression is almost completely lost in injected embryos (n=43). (C) Isolated whole gut stained for elastase expression from embryo injected with 15 ng of brunol1 morpholino and 200 pg of flag-brunol1 mRNA. Elastase expression was restored in 27/38 embryos.
Image published in: Horb LD and Horb ME (2010)
Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| cela1.2.L | elastase | X. laevis | Sometime during NF stage 42 to NF stage 49 | pancreas |
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