Fig. 2. Function of Xfurry as a transcriptional corepressor. (A) Fluorescent signals of Xfurry constructs in stage-10 mesodermal cells (Fig. S5). Red signals indicate cell nuclei stained with DAPI. (Top Row) Xfurry+GFP. (Second Row) FD+LZ+GFP construct. (Third Row) LZ+GFP. (Bottom Row) FD+GFP. Arrowheads show colocalization of GFP and DAPI signals. Arrows indicate noncolocalized signals. (B) Control embryo (stage 27). (C) Dorsal injection of FD+SiaHD interfered with head development. (D) Expression of FD+SiaHD reduced transcripts of goosecoid (Gsc) and Cerberus (Cer). (E–G) Phenotypes of injected stage-29 embryos. (E) Control embryo. (F) Ventral injection of enR+LZ (400 pg/blastomere) induced secondary axes in all embryos (n = 59). (G) Dorsal injection of VP16+LZ (400 pg/blastomere) shortened the dorsal axis in all embryos (n = 60). (H) Dorsal expression of enR+LZ induced chordamesodermal and head organizer genes, and expression of VP16+LZ reduced those genes (stage 10).
Image published in: Goto T et al. (2010)
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