Larger Image Fig. 5. Osr1/Osr2 act upstream of Wnt-mediated pulmonary specification and RA-regulated lung bud growth. (A-G) Osr1/Osr2-MO- and MM-MO-injected embryos were treated at stages 25-35 with DMSO vehicle, 10 μM BIO or 1 μM all-trans-retinoic acid (RA), and analyzed for nkx2.1, sox2, wnt2b and raldh2 at stage 35 (A-F) and sftpc at stage 42 (dorsal view) (G). Black arrows indicate normal expression, red arrows show absent expression, green arrows and lines indicate expanded expression and white arrows indicate rescued expression. Image published in: Rankin SA et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Sometime during NF stage 32 to NF stage 35 and 36 endoderm foregut lung primordium brain forebrain head pharyngeal arch eye thyroid primordium sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Sometime during NF stage 32 to NF stage 33 and 34 foregut brain spinal cord head region pharyngeal arch otic vesicle endoderm wnt2b.L wnt-2b, wnt2b-a, wnt2b-b, Xwnt-2b, xwnt2b X. laevis Throughout NF stage 33 and 34 lateral plate mesoderm lens placode pineal gland otic vesicle pharyngeal arch aldh1a2.L raldh-2, raldh2, XRaldh2 X. laevis Sometime during NF stage 33 and 34 to NF stage 35 and 36 lens otic vesicle pronephric kidney pronephric duct adenohypophyseal placode brain midbrain hindbrain sftpc.S SP-C, SPC, surfc, xSP-C X. laevis Throughout NF stage 42 lung lung primordium Image source: Published Experiment + Assay Source Phenotypes and Disease Xtr Wt + osr1 MO + osr2 MO + NF35/36 (in situ hybridization) fig.5.d Expression Phenotype decreased amount aldh1a2 expression in lateral plate mesoderm decreased amount aldh1a2 expression in pronephric mesenchyme decreased amount nkx2-1 expression in lung bud decreased amount sox2 expression in foregut decreased amount wnt2b expression in lateral plate mesoderm Xtr Wt + osr1 MO + osr2 MO + BIO + NF35/36 (in situ hybridization) fig.5.e Expression Phenotype decreased amount aldh1a2 expression in lateral plate mesoderm decreased amount aldh1a2 expression in pronephric mesenchyme increased amount nkx2-1 expression in pharyngeal region mislocalised nkx2-1 expression in pharyngeal region decreased amount sox2 expression in foregut decreased amount wnt2b expression in lateral plate mesoderm Xtr Wt + osr1 MO + osr2 MO + RA + NF35/36 (in situ hybridization) fig.5.f Expression Phenotype increased amount aldh1a2 expression in lateral plate mesoderm mislocalised aldh1a2 expression in pharyngeal region decreased amount nkx2-1 expression in lung bud decreased amount wnt2b expression in lateral plate mesoderm Xtr Wt + BIO + NF35/36 (in situ hybridization) fig.5.g Expression Phenotype mislocalised sftpc expression in foregut Xtr Wt + BIO + RA + NF35/36 (in situ hybridization) fig.5.g Expression Phenotype increased amount sftpc expression in pharyngeal region Xtr Wt + osr1 MO + osr2 MO + NF35/36 (in situ hybridization) fig.5.g Expression Phenotype decreased amount sftpc expression in lung bud Xtr Wt + osr1 MO + osr2 MO + BIO + NF35/36 (in situ hybridization) fig.5.g Expression Phenotype mislocalised sftpc expression in foregut decreased amount sftpc expression in lung bud Xtr Wt + osr1 MO + osr2 MO + BIO + RA + NF35/36 (in situ hybridization) fig.5.g Expression Phenotype increased amount sftpc expression in pharyngeal region mislocalised sftpc expression in pharyngeal region Xtr Wt + osr1 MO + osr2 MO + RA + NF35/36 (in situ hybridization) fig.5.g Expression Phenotype decreased amount sftpc expression in lung bud mislocalised sftpc expression in lung bud Xtr Wt + RA + NF35/36 (in situ hybridization) fig.5.g Expression Phenotype mislocalised sftpc expression in foregut Permanent Image Page Printer Friendly View XB-IMG-77882