Larger Image

Fig. 6. Osr1/Osr2 promotes lung specification and growth by restricting BMP signaling. (A). Analysis of Osr1/Osr2-MO and control MM-MO stage 35 embryos indicated that expression of bmp4 and BMP-target genes msx1 and hand1 are expanded into the ‘wnt2b-domain’ of the lpm on the Osr1/Osr2-depleted side. Black arrows indicate normal expression, red arrows indicate absent expression and green arrows indicate enhanced expression. (B) Confocal immunostaining of stage 35 embryos for phospho-Smad1/5/8 (green) and fibronectin (red). Scale bars: 50 μm. White arrows in B indicate ectopic pSMAD1 expression in dorsal foregut endoderm. (C) Analysis of nxk2.1, sox2, wnt2b and raldh2 at stage 35 or sftpc at stage 41 in embryos injected with Osr1/Osr2-MOs or MM-MOs and treated at stages 25-35 with either DMSO (vehicle control) or 10 μM LDN193189. Insets show ventral views of wnt2b expression. For analysis of sftpc expression at stage 41, embryos were removed from the LDN193189 at stage 35 and further cultured in DMSO vehicle until stage 41. Black arrows indicate normal expression, red arrows indicate absent expression, green arrows indicate expanded expression and white arrows indicate rescued expression.

Image published in: Rankin SA et al. (2012)

Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
wnt2b.Lwnt-2b, wnt2b-a, wnt2b-b, Xwnt-2b, xwnt2bX. laevisThroughout NF stage 35 and 36lateral plate mesoderm
lens
head
brain
pharyngeal arch
eye
otic vesicle
pineal gland
bmp4.Lbmp-4, bmp2b, bmp2b1, bmp4-a, bmp4-b, DVR-4, ofc11, XBMP-4, xbmp4, zymeX. laevisThroughout NF stage 35 and 36lateral plate mesoderm
heart
head
brain
pharyngeal arch
eye
msx1.Lhox-7, hox7, msx-1, msx1-a, msx1-b, Xhox-7.1, Xmsx-1, xmsx1X. laevisThroughout NF stage 35 and 36lateral plate mesoderm
head
brain
pharyngeal arch
eye
lens
spinal cord
epidermis
cement gland
epibranchial placode
hand1.LeHand, hand-1X. laevisThroughout NF stage 35 and 36lateral plate mesoderm
heart
pharyngeal region
head
sftpc.SSP-C, SPC, surfc, xSP-CX. laevisThroughout NF stage 42lung
sox2.Lanop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2X. laevisThroughout NF stage 35 and 36esophagus
foregut
stomach
brain
spinal cord

Image source: Published

Experiment + Assay Source Phenotypes and Disease
Xtr Wt + osr1 MO + osr2 MO + NF35/36 (in situ hybridization) fig.6.a
Expression Phenotype
increased amount bmp4 expression in lateral plate mesoderm
mislocalised bmp4 expression in lateral plate mesoderm
increased amount hand1 expression in pharyngeal region
mislocalised hand1 expression in pharyngeal region
mislocalised msx1 expression in lateral plate mesoderm
increased amount msx1 expression in lateral plate mesoderm
decreased amount wnt2b expression in lateral plate mesoderm
Xtr Wt + osr1 MO + osr2 MO + NF35/36 (immunohistochemistry) fig.6.b
Anatomical Phenotype
abnormal development of foregut
abnormal foregut morphology
Expression Phenotype
increased amount smad1 expression in dorsal foregut
Xtr Wt + LDN-193189 + NF35/36 (in situ hybridization) fig.6.c
Expression Phenotype
increased amount aldh1a2 expression in lateral plate mesoderm
increased amount sox2 expression in foregut
mislocalised sox2 expression in foregut
increased amount wnt2b expression in lateral plate mesoderm
Xtr Wt + osr1 MO + osr2 MO + NF35/36 (in situ hybridization) fig.6.c
Expression Phenotype
decreased amount aldh1a2 expression in lateral plate mesoderm
decreased amount nkx2-1 expression in lung bud
decreased amount sox2 expression in foregut
decreased amount wnt2b expression in lateral plate mesoderm
Xtr Wt + osr1 MO + osr2 MO + NF42 (in situ hybridization) fig.6.c
Expression Phenotype
decreased amount sftpc expression in lung bud

Permanent Image Page
Printer Friendly View

XB-IMG-77883