Fig. 4. Phenotypes of ptf1a/p48-TALEN targeted X. tropicalis. (A and B) Anatomical analysis revealed visceral abnormalities such as much reduced pancreas in ptf1a/p48-TALEN-injected G0 froglets. The pancreas is outlined by dashed lines. (C) DNA sequencing of genomic DNA extracted from hindlimb tissue dissected from a froglet showing a phenotype similar to that in B confirmed the gene disruption at the ptf1a/p48 locus (20/23) (du, duodenum; st, stomach). (D) Whole-mount in situ hybridization of pancreas marker pdip in X. tropicalis tadpoles injected with the indicated TALEN mRNAs. ptf1a/p48-TALENs, but not ets1-TALENs, induced repression of pancreas bud formation. (D) Uninjected control embryos, (E) ptf1a/p48-TALEN- injected embryos (800 pg), and (F) ets1-TALEN-injected embryos (800 pg). (G) Summary of the phenotypes shown in D. The TALEN mRNAs were injected into the animal pole region at the one-cell stage; embryos were analyzed at stage 40.
Image published in: Lei Y et al. (2012)
Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| pdia2 | pdi, pdip, XPDIp | X. tropicalis | Throughout NF stage 40 | pancreas dorsal pancreatic bud ventral pancreatic bud |
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