Figure 3 Novel retinal stem cell markers retrieved from the screen. A: Stage 39 retinal sections showing the specific expression in retinal stem cells of the 18 clones isolated from the screen (gene symbol is indicated on each panel). A'': Magnification of the corresponding dorsal CMZ (except for sulf2 in (B0) for which the ventral CMZ is shown). White doted lines delineate the staining. S: Quantification of the staining extent in the dorsal CMZ. Values represent the stained surface relative to total CMZ area and are given as mean +/- SEM (n = at least 6 retinal sections per clone). Note that although all clones are expressed in the stem cell containing region of the CMZ, staining expansion differs from one to another (light grey: less than 25% of the CMZ area; medium grey: between 25 and 45%; dark grey: more than 45%). In addition, major dorso-ventral variability is observed for sulf2, which was thus quantified in both the dorsal and ventral CMZ as indicated. Scale bar = 50 um. Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com. This material is reproduced with permission of John Wiley & Sons, Inc.
Image published in: Parain K et al. (2012)
Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| alpl | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone | |
| sulf2 | xtsulf2 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| spry4 | sprouty 4, sprouty-4, sprouty4 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| gxylt1 | glt8d3 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| mgrn1 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone | |
| hes4 | H2, hairy2, hairy2a, hairy2b, hes4-a, hes4-b, hes4a, hes4b, Xhairy2, XHairy2a, Xhairy2b | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| nop58 | nop5, nop5/nop58, nop58-a, nop58-b | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| mhc1-uda | class Ib16004, LOC100127707, LOC108711789, LOC121402286, XB5873923 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| ranbp1 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone | |
| kpnb1 | imb1, impnb, importin beta, ipo1, ipob, LOC108700788, ntf97 | X. tropicalis | Throughout NF stage 39 | stem cell |
| aurka | aik, ark1, aur-a, aura, Aurora A, Aurora-A, aurora2, btak, Eg2, pEg2, stk15, stk6, stk7 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| ncapg | capg | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| zmcm3 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone | |
| mcmbp | MGC79802 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| ssbp1 | mt-ssb, mtssb, soss-b1, ssbp1-a, ssbp1-b | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| trmt2a | htf9c | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| dnajc27 | dnajc27-a, dnajc27-b, rabjs, rap1, rbj1 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
| fndc3b | fad104, pro4979 | X. tropicalis | Throughout NF stage 39 | stem cell ciliary marginal zone |
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