Fig. 1. Cloning and expression analysis of xBtg-x. (A) The �combined organizer graph� of clones regulated by maternal β-Catenin (x axis) and Nodal-related signaling (y axis) in a cDNA macroarray screen (Wessely et al., 2004). Each spot represents one cDNA clone analyzed. The positions of xBtg-x, Xnr-3 (a target of β-Catenin), and chordin (a target of both β-Catenin and Nodal-related signaling at gastrula) are indicated. (B) Schematic diagram of xBtg-x showing the conserved Boxes A and B of the antiproliferative domain characteristic of members of the Btg/Tob gene family. (C) Phylogenetic tree of the Btg/Tob gene family. Alignment was performed using the ClustalW program. (D) RT-PCR analysis of xBtg-x and chordin expression in stage 4 to stage 17 Xenopus embryos. Ornithine Decarboxylase (ODC) serves as loading control. (E�L) Whole-mount in situ hybridization of Xenopus embryos with xBtg-x antisense probe. (E�H) Expression of xBtg-x in gastrula stage embryos viewed from the vegetal side with dorsal to the top: (E) uninjected control; (F) embryo treated with LiCl at the 32�cell stage; (G) embryo injected with 800 pg δN-XTcf-3 mRNA, or (H) 150 pg of the Nodal-related antagonist cer-S mRNA. (I) Expression of xBtg-x at stage 9, lateral view; (J) stage 10 hemi-sectioned along the dorso-ventral axis, dorsal to the right; (K) stage 14 and (L) stage 15 in dorsal view, anterior to the left.
Image published in: Wessely O et al. (2005)
Copyright © 2005. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| btg5.2.S | xbtg-x | X. laevis | Throughout NF stage 9 | dorsal marginal zone animal hemisphere dorsal animal |
| btg5.2.S | xbtg-x | X. laevis | Throughout NF stage 10 | dorsal marginal zone endomesoderm dorsal ectoderm |
| btg5.2.S | xbtg-x | X. laevis | Throughout NF stage 14 | neural plate posterior neural crest trunk neural crest cranial neural crest chordal neural plate border |
| btg5.2.S | xbtg-x | X. laevis | Throughout NF stage 15 | chordal neural plate border posterior |
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