Figure 3. Turbulent Flow in ATP4a Morphants Causes Bilateral Nodal Cascade Induction(A�C) Analysis of bead trajectories in time-lapse movies of dorsal explants from representative (A) CoMO-injected or (B) ATP4aMO-injected embryos and (C) specimen treated with SCH28080. Flow is displayed as GTTs of 25 s length; cf. color bar in (A). Note that trajectories in (B) and (C) project to the left side (indicated with blue arrows) and right side (indicated with pink arrows) of the GRP, whereas GTTs in (A) point uniformly to the left. White arrows represent trajectories running anteriorly or posteriorly.(D) Whole-mount in situ hybridization of stage 20 dorsal explants with probes specific for (top) Coco and (bottom) Xnr1. Left-sided Coco repression was lost in (middle) ATP4a morphants and inverted upon (right) parallel left-sided knockdown of flow. Xnr1 expression was unaffected.(E) Quantification of Coco expression patterns.(F) Quantification of Pitx2c expression patterns in stage 26�32 tadpoles following MO injections into the C1 lineage (dorsal midline-GRP) of four-cell embryos as indicated. Note that the bilateral induction (cf. Figure 1F) in ATP4a morphants was dependent on both the presence of GRP-Xnr1 and ciliary motility.See also Movie S1.
Image published in: Walentek P et al. (2012)
Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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