Figure 3. The mutation of a second tyr gene target site also caused albinism. (a) Phenotype of injected embryos (bottom five embryos; top five embryos are uninjected sibling controls) with Cas9 mRNA (2.2 ng) and sgRNA (200 pg) for the second target site. (b) Representative mutation profiles from two embryos sequenced after cloning. The second target sequence (shaded in purple) and the PAM sequence (in red) are shown together with first target and PAM sequences (in gray; see Fig. 2) relative to the ATG (Met) site. The alignment shown is labeled as described in Fig. 2b
Image published in: Nakayama T et al. (2013)
Copyright © 2013. Image reproduced with permission of the Publisher, John Wiley & Sons.
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