Figure 1. CRISPR/Cas targeting of the tyrosinase locus in Xenopus tropicalis. (a) Cas9-bound gRNA:DNA target region on the tyr gene. The 5′→3′ orientation of the transcription unit is from right to left. Overlap with the TALEN binding sites (boxed in yellow) from Ishibashi et al. (2012) is shown. (b) Right panel contains examples of stage 41 embryos injected with hCas9 and tyr gRNA. These tadpoles exhibit typical oculocutaneous albinism found in most of the embryos. A subset retained more pigment but significantly less than wild-type levels (not shown). Left panel is a wild-type control sibling from the same clutch.
Image published in: Blitz IL et al. (2013)
Copyright © 2013. Image reproduced with permission of the Publisher, John Wiley & Sons.
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