Fig. 5. Spatial expression of E59 (FGFR-Al), G62, and E30 transcripts in tailbud Xenopus embryos. Whole-mount in situ hybridizations were performed on albino Xenopus embryos using digoxigenin-labeled antisense RNA probes. Hybridization signals were visualized as alkaline phosphatase chromogenic reaction products. A: Lateral view of a stage 29/30 embryo stained for E59 mRNA expression. E59 transcripts are detected mainly in the brain, pronephros, and somites. Note also the high levels of expression in unsegmented somitogenic mesoderm (sm). 9: Close-up view of A at higher magnification showing details of E59 mRNA expression in the anterior half of the embryo. Extensive expression is seen in the fore-, mid-, and hindbrain (fb, mb, and hb). The otic vesicle (ov) is indicated as a reference point for the posterior limit of the E59 expression in the hindbrain. Staining is also associated with visceral arches (arrows), the pronephros (pn), and the pronephric duct (pd). C: Lateral view of a stage 29/30 embryo stained for G62 mRNA. Similar to E59, G62 transcripts are mainly detected in the brain, pronephroi, and somites. No expression was detected in unsegmented somitogenic mesoderm as with E59. D: Close-up view of C at higher magnification. In the brain, expression of G62 transcripts is seen predominantly in the eye vesicle (ev), midbrain (mb), and hindbrain (hb). The midbrain/hindbrain junction (j) is devoid of G62 transcripts. Other areas of expression include the pronephros (pn), the pronephric duct (pd), and the tissues in the region of the liver rudiment (Ir). Faint expression is also seen in the visceral arches (arrows). E: Lateral view of a stage 28 embryo stained for E30 mRNA expression. Extensive expression of E30 transcripts is seen in the brain, visceral arches, and at lower levels in somites. F: Close-up view of E at higher magnification to illustrate staining of the olfactory placode (op), the wall of the otic vesicle (ov), the forebrain (fb), and visceral arches (arrows). All embryos were rendered transparent with Murray's clear prior to hybridization. Anterior ends of embryos are oriented to the left. Scale bars: A = 288 pm; B = 144 pm; C, E = 225 km; D, F = 112.5 pm.
Image published in: Brändli AW and Kirschner MW (1995)
Copyright © 1995. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| fgfr1.L | bfgfr, cek, fgfr-1, fgfr1-a, fgfr1-b, flg, flt-2, kal2, ogd, X1FGFR, XFGFR-1, xfgfr1, xfgfra2 | X. laevis | Throughout NF stage 29 and 30 | pharyngeal arch mandibular arch hyoid arch branchial arch brain forebrain midbrain hindbrain presomitic mesoderm otic vesicle pronephric kidney pronephric duct eye |
| fgfr4.S | FGF receptor 4, fgfr-4, fgfr4-a, fgfr4-b, XFGFR-4, XFGFR-4a, xfgfr4 | X. laevis | Throughout NF stage 29 and 30 | pharyngeal arch brain forebrain midbrain hindbrain pronephric kidney pronephric duct foregut liver primordium liver otic vesicle optic vesicle eye |
| ptk7.L | cck-4, cck4, klg, ptk-7 | X. laevis | Throughout NF stage 28 | pharyngeal arch brain forebrain otic vesicle olfactory placode hypothalamus |
Image source: Published
Permanent Image Page
Printer Friendly View
XB-IMG-84561