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Figure S2. Analysis of arteriogenesis and vasculogenesis in Eto2-MO and Eto2-MO2 injected embryos, related to Figures 2, 3. Expression analysis of the arterial marker Cx37 (A, stage 34), the endothelial genes VE-cadh, Ami, Fli1 and AA4 (B-C, stages 37/34), Flt1 and Flt4 (D, stage 26) in Eto2-MO and Eto2-MO2 morphants. Red arrowhead, DA; black arrow, PCV; yellow arrow, trunk vasculature; green arrowhead, DLP. (C) Uncleared embryos, bracket indicates intersomitic vessels (ISVs) sprouting from the PCV in wild-type embryos; the graphs show the number of ISVs observed in wild-type, Eto2-MO and Eto2-MO2 embryos. Numbers at the bottom of the panels indicate the number of embryos with the given phenotype out of the total number examined. Whole mounts are shown with anterior to the left, dorsal to the top.

Image published in: Leung A et al. (2013)

© 2013 ELL & Excerpta Medica. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license

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