Larger Image Fig. 3. Co-expression of XIF3 and neural specific tubulin with antibody markers for primary neurons. (A-D) Transverse sections of stage 22 embryos at two positions along the A-P axis: (A and C) embryos at stage 22 stained by in situ hybridization with an DIG-labeled antisense RNA probe to XIF3 (blue) and then by whole-mount antibody staining with anti-HNK- 1(brown). (B and 01 Sections stained by in situ hybridization with a neural specific tubulin (NST) specific antisense probe (blue) and then by whole-mount antibody staining with anti-HNK.1 (brown). At this stage the antibody stains a structure within the cell that is probablv the Goigi apparatus. Note the co-localization of the two stains in the primary neurons. In addition XIF3 staining is seen in undifferentiated neuroectoderm at more rostral levels (panel A). Image published in: Goldstone K and Sharpe CR (1998) Copyright © 1998. Reproduced with permission of the Publisher, University of the Basque Country Press.
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