Larger Image

Fig. 5. Triple PG1 knockdown severely affects hindbrain patterning. Morpholinos for each of the Hox PG1 genes (A1/B1/D1MO) were co-injected into the lhs of the embryo (B,D,F,H,J). Non-injected controls are also shown (A,C,E,G,I). The hindbrain region is shown, with anterior to the top. Expression of the neural marker Nrp1 (A,B: 100%, n=11), the anterior marker Otx2 (C,D: 100%, n=10), Gbx2, which is expressed in r1 (E,F: 77%, n=13) and Krox20 (G,H: no stripes 41%; one faint stripe 35%, n=71), was analysed. Dotted line indicates the midline. In later embryos (st. 46), the neural antibody 2-G9 was used to show the morphology of the hindbrain [NIC, I; A1/B1/D1MO, injected on the left-hand side (lhs) *, J: 100%, n=5]. Rhombomere boundaries are marked.

Image published in: McNulty CL et al. (2005)

Copyright © 2005. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
nrp1.LA5 antigen, np-1, neuropilin-1, nrp, npn-1, nrp-1, neuropilin, pNPG152X.laevisThroughout NF stage 22neural plate
midbrain
hindbrain
neural tube
posterior neural tube
anterior neural tube
forebrain
telencephalon
diencephalon
spinal cord
eye
otic placode
optic vesicle
otx2.SXotx2, otx-2, Xotx-2, otxA, otx2-a, otx2-bX.laevisThroughout NF stage 22anterior
eye
optic vesicle
forebrain
cement gland primordium
gbx2.1.SXGBX-2X.laevisThroughout NF stage 22hindbrain
midbrain-hindbrain boundary
cranial neural crest
rhombomere
otic placode
egr2.LXKr20, EGR-2, Krox-20, XKrox-20, krox20X.laevisThroughout NF stage 24hindbrain
cranial neural crest
rhombomere R3
rhombomere R5
anterior branchial crest
myod1.Smyogenic factor 3, MyoD, XtmyoD, XMyoD, mf25, myod1-b, myod1-aX.laevisThroughout NF stage 22somite
muscle
myocardium
lsamp.Llamp, iglon3, 2G9X.laevisThroughout NF stage 46midbrain
hindbrain
neural tube
rhombomere
rhombomere R2
rhombomere R3
rhombomere R4
rhombomere R5
rhombomere R6
brain
forebrain

Image source: Published

Permanent Image Page
Printer Friendly View

XB-IMG-46055