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XB-PHENO-22997

Xla Wt + ctnnb1del + sox17b.1 + ctnnb1 MO + animal cap explant, Sinner D et al. (2004)

Experiment + Assay: Xla Wt + ctnnb1del + sox17b.1 + ctnnb1 MO + animal cap explant + gastrula stage (RT-PCR)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection sox17b.1.L sox17b.1.L
sox17b.1.S
 NF stage 2 (2-cell) blastomere
mRNA injection Tg(ctnnb1del)Rtmn ctnnb1.L
ctnnb1.S
 NF stage 2 (2-cell) blastomere
morpholino injection ctnnb1 MO1 ctnnb1.L
ctnnb1.S
 NF stage 2 (2-cell) blastomere
explant  
NF stage 8 animal cap
Conditions:
Assay Type Assay Stages Antibody
RT-PCR gastrula stage  
Expression Phenotypes (4):
increased amount a2m.L expression in animal cap
increased amount foxa1.L expression in animal cap
increased amount foxa2.L expression in animal cap
increased amount hnf1b.L expression in animal cap
Source: fig.7
Article: XB-ART-3496, PubMed