Dr. Judith L. Campbell
Professor of Chemistry and BiologyDivision of Biology and Biological Engineering
Mechanisms and Regulation of DNA Replication and Repair The Campbell Group studies DNA replication in the yeast Saccharomyces cerevisiae. Yeast offers an as yet unparalleled ability to combine genetic, molecular, and biochemical approaches in a eukaryotic organism. In the past, biochemistry has focused on the characterization of individual proteins. In modern biochemistry, the emphasis is on studying protein/protein interactions in multienzyme assemblies. In keeping with this trend, our overall goal is to reproduce the assembly and activation of the replication fork at an origin of replication using purified proteins. Our work in preparation for achieving this goal has ranged from defining the structure and activity of chromosomal replication origins, to the discovery of replicative enzymes, verification of their in vivo roles using yeast genetics, and the detailed characterization of their enzymatic mechanisms.