| Description |
Xenopus is the leading vertebrate model for the analysis of gene function in development. The combination of lineage analysis, gene-knockout strategies, experimental manipulation of the embryo, and genomic/bioinformatic techniques, makes it ideal for \
studies on the molecular control of embryo patterning, morphogenesis and organogenesis. Moreover, recent advances in Xenopus genomics offer new opportunities to integrate computational strategies with experimental approaches. The course combines intensive labora\
tory training with daily lectures from recognized experts in the field. Students will learn both emerging technologies and classical techniques to study gene function in Xenopus development. An important element will be the informal interaction between students and cou\
rse faculty.
Technologies to be covered will include: oocyte and embryo culture, lineage analysis and experimental manipulation of embryos, time lapse imaging of morphogenesis, gain and loss of function analysis using mRNAs and antisense oligonucleotides, whole mount in si\
tu hybridization, immunocytochemistry, genomics and bioinformatics, chromatin immunoprecipitation, preparat
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