On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development
Peshkin et al., 2015, Developmental Cell 35, 383–394 November 9, 2015
A biochemical explanation of development from the fertilized egg to the adult requires an understanding of the proteins and RNAs expressed over time during embryogenesis. We present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most protein levels change little and duplicated genes are expressed similarly. While the correlation between protein and mRNA levels is poor, a mass action kinetics model parameterized using protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides detailed data for absolute levels of ∼10,000 proteins and ∼28,000 transcripts via a convenient web portal, a rich resource for developmental biologists. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the dynamics of the embryonic proteome.
Follow this link: http://kirschner.med.harvard.edu/MADX.html to query the data from the Kirshner lab.
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The paper can be accessed free through Dec. 29, 2015 by following this link: http://authors.elsevier.com/a/1S0Dp5Sx5gPQfn
Or, can be accessed from Developmental Cell here: http://www.sciencedirect.com/science/article/pii/S1534580715006577
Last Updated: 2015-11-10