XB-CLONE-12441199
|
Clone Name: pax8-475
|
Gene:
|
Species: Xenopus laevis
|
|
|
Description: EXRC Clone Number 475.
Xenopus laevis paired box 8 (pax8)
To generate probe for in situ and mRNA for injection.
The partial Pax8 cDNA was isolated from Xenopus adult kidney
|
Sequence:
Full length sequence: pBS.KLPax8.1_FullLength
[+]
cgGncCCCtcngtTTTTTTTTTTTTTTTTTCTTtctGCTTaTTTaTTTaAAATATTTTATATtACAAATATttaTttaCagtctGGCCctgtagtTGctg
TATTTCCACAAACAGGACCAtgCGaTAAAGTCATTTagtGCGgagCAGGCACAATCCTgtAATAATGCctgATcGTAAGtCTTTCCTTCATTCAAACctg
tTGCATTTTATACCCCCAGcacttgAgtGCACTCAGCCgtCAcgtGGgaAGTagaATATAAAATATGCCTgtgTCCAanaACAAGTAATTAGAGACACTg
tgtGCTGAGGCCCCAATCAAAAATTAATTCagaAATGTCCAACCTTCAGCTCAATTGGTTAACAgAAAGCAACGGGGTTGACTTAATAACATGGGTATTA
ACATGTCCCAGAGCCGTTACCAGTAACAGCCAATAagaACTTTTCATTTCTAATGTGTTAGACTGATCACAGTTAATCATTGGCTGGTTGCTATGGGCGA
CTACCTACAGTGGGCCATTTTAGCACCTTTGTTAGTAAATTAGCCATGCTGTGTTTGTGCCACCTTCTTCAAAGTCCCGCTTTCATGACTGAATGTGAAC
CGACACTAATAGTATAAATAATTAGTGCATTCAAATCACTTTTAAAGCCCTTGTGGCTACATCAACAGTATATAGAGATCTGCATAGATGTATCTGTTTT
ACATACATACATAAATATATACAGTATAGTTGTGTAGTCTTTGCCCCTGCAACTGTGCAGGGAGTGGTAGTACCACAACACCAAGATCTCCCCTCTCTCC
TCTGTACAAGTACTTGAATGCAAATCCATTGGACAGCTTTGggAAAATCCTGCTTTCTAAAAGGGAAAGGGTTGCAGGCTCATCCCTTTAAAAGTGGACA
GATATCAAATCCAATGGCTTATTAACAAATTATTTTGGAGGCCGTACTGGcCCACCGTGATACCAggataACTCCCGGTgGGcCCAGGTGTCAATgGGCT
ACTGCATCTAACCTATAGTATTTTATgGTCATGCCCTaTTACTgtaTGGAacaATGAGGCTGAATagaTGGAanaaTAGGAtAtAGtACATAAAgGGAag
tGactana
Construction:
Insert:
|
| Size: 3000 bp | Source Tissue: kidney | Stage: |
Vector:| Vector Details | Vector Map |
Name: pBluescript II SK-
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
|
RNA injection:
|
|
|
| |
|
Probes:
|
| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
|
T3 |
XhoI |
|
|
|
Whole mount in situ hybridization:
|
Expressed on Pronephros and duct, CNS.
|
|
| |
|
Probes:
|
| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
|
T3 |
EcoRI |
|
|
Suppliers:
Search for clone at:
EXRC
