Summary Attributions

Clone Name: shh-HAR18	Gene: shh	Species: Xenopus tropicalis
Description: EXRC Clone Number HAR-18. TNeu067o14. Xenopus tropicalis sonic hedgehog. Not sure of length. 3' present. To generate probe for in situ.

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Sequence:

5' EST: AL804833 [+]

aatccccggggtcggaccgggaccgcagtaaatacgggatgctgggcaggctggcggtggaagccgggttcgactgggtctattttgagtccaaggctca
catccactgctcggtcaaagcagagaactcggtggcggccaagtcgggggggtgcttcccgggcagtgccagggtgatggtggaaccgggcggcaccaaa
gcggggagagagctgcgccccggggaccgggtcctctcctccgacccccaagggaatctcatctacagcgatttcctcctgttcatagacaaggagcatg
acgtcaagaagctctattacgtcatccagacgtctcagacccgaatccggatgacggcggcccacctgcttttcgtggcccagagcaacggaaccgggtc
tttcaagtctgtgtttgccagcaacgtccgacctggggatgtcatttatagcgcagatcggagggatatgaccttgagggaggcgatggtggagaaagtt
gaccttgaggaggacattggggcttttgcccccgtaactgc

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Library:

Name: XGC-neurula

External Dbs: Unigene tropicalis cDNA library

Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from neurula. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

Anatomy: whole organism	Stage: NF stage 13 to NF stage 21
Normalized: No	Subtraction: No

Vector Details	Vector Map
Name: pCS107 Type: plasmid 5' Restriction Site: EcoRI 3' Restriction Site: NotI

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Usage:

Whole mount in situ hybridization:
Probes:
Type Size Promoter Linearization Site Description antisense T7 BamHI

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Suppliers:

Search for clone at: EXRC