XB-CLONE-12440896
Clone Name: t-366
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number 366. T, brachyury homolog
For the synthesis of RNA and inhibition of Xbra
The construct was geneated by destroying the initial methionine in engrailed, derived from the plasmid PMEnT (Badiani et al) and replacing it with a Cla 1 site. The construct is myc tagged via HindIII and a short oligo links myc to the engrailed repressor.
The DNA biding domain of Xbra (up to Cla1 site) fused to the engrailed repressor and a myc tag
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Sequence:
Full length sequence: PSP64T Xbra-EnR_FullLength [+]
tnACGCTCAnCTTTGGCaGaTCaTTCCGAAAGGAATACGAGTCAcTACCTGCCGATCACTCGCACCTTGGGtTTTGTTCCGATTACAATAAAACAAATCA
GTGGAAAAGCTGCTGTAAGtTTTTTTTGCCCCTGTTTGTGTGTTTGGACCCCCCTCTCTGGAGTAATGAGTGCGACCGAGAGCTGCGCCAAGAACGTACA
GTACCGGgtGGATCATCTTCTCAGCGCTGTGGAGAATGAGCTCCAGGCTGGCAGCGAGAAGGGGGACCCCACCGAGAAGGAGCTGAAGGTTAGCCTGGAG
GAGCGGGACCTGTGGACGAGGTTCAAGGAGCTCACCAACGAGATGATCGTCACCAAGAATGGAAGACGAATGTTTCCAGTTCTGAAGGTGAGCATGTCGG
GCCTGGATCCCAATGCAATGTATACGGTTCTGCTGGACTTTGTGGCAGCCGACAACCACCGCTGGAAGTATGTGAATGGAGAATGGGTTCCAGGTGGCAA
ACCCGAGCCCCAGGCCCCCAGTTGTGTTTATATCCACCCAGACTCACCCAACTTTGGAGCCCACTGGATGAAAGATCCTGTCTCTTTTAGCAAAGTCAAA
CTTACAAACAAAATGAATGGTGGAGGCCAGATTATGTTAAACTCTTTGCACAAGTATGAACCTCGAATCCACATAGTGAGAGTTGGTGGCACCCAGAGAA
TGATCACTAGCCATTCATTCCCTGAGACACAGTTCATAGCAGTGACCGCATACCAGAATGAAGAGATTACAGCACTTAAAATCAAACACAATCCATTTGC
CAAAGCATTTCTTGATGCAAAAGAAAGAAATGATTATAAAGACATCTTGGATGAGGGAATCGATGCCCTGGAGGATCGCTGCAGcCCACAGTCAGCGCCC
AGcCCCATTACCCTACaAATGCAGCATCTTCACCACCAGCAACAGCAGCAGCAGCAACAGCAGCAGCaAATGCAGCACCTCCACCAGCTGCAGCAACTGC
AGCAGTTGCaCCAACAGCAACTggccgcCGGTGtcttCcacCATCCGgcaATGGcntTCGaTgCcgCTGcagCCgccGCTGCtg
Construction:
Insert:
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Size: | Source Tissue: | Stage: |
Vector:Vector Details | Vector Map |
Name: pSP64T
Type: plasmid
5' Restriction Site: null
3' Restriction Site: null | |
Usage:
RNA injection:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
sense |
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SP6 |
EcoRI |
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Suppliers:
Search for clone at:
EXRC