Summary Attributions

Clone Name: smad1-524	Gene:	Species: Xenopus laevis
Description: EXRC Clone Number 524. Xenopus laevis SMAD family member 1 (smad1-a). Very close in sequence to XMad1 of Graf et al., Cell 1996, but some nucleotide and amino acid differences. Possibly Xmad and XMad1 are alleles. For Wish and RNA Injection.

---

Sequence:

Full length sequence: pXMad_FullLength [+]

aTcCaTCGaTTTAnGCTaTGGaGCnAAAGCTCATTTCTGAAGAGGACTtgAATGAAATGGAGCAAAAGCTCATTTCTGAAGAGGACTTGAATGAAATGGA
GCAAAAGCTCATTTCTGAAGAGGACTTGAATGAAATGGAGCAAAAGCTCATTTCTGAAGAGGACTTGAATGAAATGGAGCAAAAGCTCATTTCTGAAGAG
GACTTGAATGAAATGGAGAGCTTGGGCGACCTCACCATGGAGCAAAAGCTCATTTCTGAAGAGGACTTGAATTCAAATGTGACAAGCCTTTTCTCCTTCA
CAAGCCCAGCTGTGAAGAGGCTGCTTGGTTGGAAACAGGGAGACGAAGAAGAGAAATGGGCAGAGAAAGCGGTGGATGCCTTGGTGAAAAAGCTGAAGAA
GAAAAAAGGAGCCATGGAAGAACTTGAAAAGGCCCTGAGTTGTCCTGGACAGCCCAGTAACTGTGTCACCATTCCTCGTTCCTTGGATGGCCGACTGCAA
GTGTCTCACCGTAAGGGCCTACCGCATGTCATTTATTGCAGAGTGTGGCGTTGGCCTGATCTACAGAGTCACCATGAACTGAAACCCTTGGAGTGTTGCG
AGTATCCCTTTGGTTCCAAACAGAAGGAGGTCTGCATCAACCCATATCATTATAAACGAGTGGAGAGTCCTGTCTTGCCGCCTGTCCTTGTTCCACGGCA
CAGTGAGTACAACCCTCAACACAGTCTCCTTGCGCAATTCCGAAACTTGGAGCCAAGTGAGCCACACATGCCTCACAACGCAACTTTTCCCGACTCTTTC
CAGCAGCCAAACAGCCATCCGTTCCCTCACTCGCCCAACAGCAGCTACCCAAACTCTCCGGGAAGCAGCAGTACTTACCCTCACTCACCGGCGAGCTCTG
ATCCTGGAAGCCCTTTTCAAATACCAGCTGACAcCCCTCCTCCAGCTTATATGCCTCCTGAGGATCAGATGACGCAAGACAACTCTCAGCCGATGGACAC
aAATATGATGGTGCCTAACATCTCTCAAGATATcaaTAGAGCAGATGTacAannTgtTGCCTATGAagagcCAAAACactGGTGCtccATTgTCTatTAT
GAGCTGaACAACCGTGtanganaa

---

Construction:

Insert:

Size: 3000 bp	Source Tissue:	Stage:

Vector:

Vector Details	Vector Map
Name: pCS2+ Type: phagemid 5' Restriction Site: null 3' Restriction Site: null

---

Usage:

RNA injection:
Probes:
Type Size Promoter Linearization Site Description sense SP6 NotI

Whole mount in situ hybridization:
Probes:
Type Size Promoter Linearization Site Description antisense T3 EcoRI T7 can also be used

---

Suppliers:

Search for clone at: EXRC