XB-CLONE-12442420
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Clone Name: sox9-KL25
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Gene:
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Species: Xenopus laevis
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Description: EXRC Clone Number KL-25.
Xenopus laevis SRY (sex determining region Y)-box 9.
To generate probe for in situ and mRNA for injection.
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Sequence:
Full length sequence: Xsox9 pGEMT_FullLength
[+]
GTTGGGAGCTCTCCCATATGGTCGACCTGCAGGCGGCCGCGAATTCACTAGTGATTACGGGTTGTTCCCAATGCTGTGGGCTGTGTGTCTGGGGGATTGA
TGGAACTCCTGTTGTGTCTGCAATAGGGGTGTACAAGGGGCGTTGGCTTGGATTCATGTAGCTAAAGGTGGAGTAGAGACCAGAATTCTGGCCACTTGCA
TGACTGTAATAGGTACTAGAACCCTGGTGCTCGGTGTAGTCATACTGTGCACGGGTGATAGTTGGGTATGAAGAGCTGTAATGCTGCAGGTTGAAGGAGC
TGTAGTTCAGCTGCTGGGGGGAATGCTGTTGCTGTTGGTCACTGTAATGACTCGGACTCAGTTGCTCGGTCTTGATGTGTGTCCTTTGCTGGGACTGGCT
TTGCTCGCTGTTTAGGGTTGACAGTGAATGTTGTTGAGGCTGCTGTTGCTGTTGTTTAGACATACAGGCAGGGCCAGCACCTGTGGTTGCACTAGGGGTG
CTGCTGATGCCATAACTGCCTGTGTACGAGGCCTGAGTGGAGCCAACCCCTGGGTGGCCATTGGGCGGCAGGTATTGGTCAAATTCATTGACATCAAAGA
TTTCAATGGTGGAGATGACCTCACTGCTCAGCTCACCAATGTCCACATCTCGGAAATCAATGCGAGGTGGCTGCCTACCATTCTCTTGCAGTGGCCTGCC
CTCCCTCTTTAGGTCTGGCTTTCCAGGCTGGATATCTGTCTTGGGAGTAGTTGGAGGAGTTGGGGGGCCTTGGGATTGACCTGAATGTTCTCCAGGAGAG
TGGACTTCGCTCATGCTGGAAGAGGAATGCGGGGAGTCAGCCTGTANGGCCTTGAAAATTGCATTAGGGGAGATGTGGGTTTGCTCAGCACCATCCTCTT
GTTCTGTCTGCCCATTCTTAACGGACTTTCTGCGCCGTGGCTGATACTTGTAGTCNGGATGATCCTTCTTGTGTTGGACCCTCANCCTCTCTGCTTCCTC
CACGAAAGGGCGTTTCTCACCCTCATTCAGAAGTCTCCATAACTTTCCCAGCGTCTTGCTGAGTTCTGCATTGTGCNNNGGGGTACTGATCAGCCAGCTT
CCTNCTTGCAGCCTGTGCNCAGACCATGAANNNTTCATGNNCNNNTGACATGGGCTTGTTNTGCTGGATCCATTANTCNGACTGGCAT
Construction:
Insert:
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| Size: 1400 bp | Source Tissue: | Stage: |
Vector:| Vector Details | Vector Map |
Name: pGEM-t-easy
Type: plasmid
5' Restriction Site: null
3' Restriction Site: null |  |
Usage:
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RNA injection:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
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T7 |
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Linearize with SocI. |
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Whole mount in situ hybridization:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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SP6 |
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Linearize with NcoI. |
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Suppliers:
Search for clone at:
EXRC
