XB-CLONE-12442424
|
Clone Name: xa-1-KL44
|
Gene:
|
Species: Xenopus laevis
|
|
|
Description: EXRC Clone Number KL-44.
Xenopus laevis anterior and ectodermic-specific protein
full length XA-1.
To generate probe for in situ and mRNA for injection.
|
Sequence:
Full length sequence: pXA-1_FullLength
[+]
NNNNNNNNNNNNNNNNNNNTNNGCGGNNNNNNNNNNNNNNNNNNNTNNNNNNNTTTGANNAGAGATANACGGGATTTCCAGCCATGTTTTTCTACGTTCT
TCTGCTTGCTCTAATGGCCCAGGGCTGGAGCCTTCCACAAGGGAAGACAGGAGAAGATTCTCCAGTCTTCAGGCCTCCTTCTCCACCCATGGGCCCTAGC
CTTCCTCCACCTGTCAGCCATGACCTGCACAGGCCATCGGGTCACCCTGAGGAGTTCAGAACTGGAGCATCCTTACCTCCAAAAGAAACCCCCAATGAAC
CGAGGCATGGCAGGCCTAAGAGAGATCTTCATCATGGGAAAGTTGTCCCCACTGGAGTCCCTCATCACACAGGAGAAGTCCTACATCACACAGACTGTTC
TTCTAATACCCACAAGTCACATGAAGAAAACAGACCTAAAGGGTTCAGGACTGGACGTCCCTTGCTACCCATAAAACCTGAGCATGGCAGGCACAGGAGA
GATCTTCATCATGGGAAAGCTGTCCCTACTGGAGTCCCTCATCACACAGAAAAATTCCACAACGGCAGCAATGGAAAAAGCCATCCTCCCCGACCAGGCC
ATTCCACCTCTGCCCACAATGACAACTCAAGTGAAGAAAAGAGACCAAAACATGGTCAGGAACAAGGAAAGAAGCATTAGTAAACATAAAAAGAAGAAAA
TTAAAGAGGAAACCGAGTACATTGGATGGAATTATTAATAAGTTATGGCAAAACCAATAAAGTGATAATGANNNAAAAAANNNAAAANANAAAAAAAAAA
NAAAAAAAAANNNNAAATNAANATAANNANATANNAANANAACAAAANAGGGANCNGGANTTCNNTATCNGTTTATCNATCCCNNCNNCCTNNNGGGGGG
GGCCCCGGTACCCANTTCCCCCNANAGGGNGNCNNATTACAATTTNNNNGGCCNNCCTTTTNNNANCGCCNTGCCGGGGAAAACCCCTGGGTTTTCCCCA
ATTATANCCCCCTTGGANAACTNCCCCCTTTTCCCCNNNNGGGGTAAAAACNAAAAGNNCCCNNCCCANNTCGCCCNTCCNAANNNTNGNCNAACCCTGA
NATGGNNAAAGGGAAAANTNGNAAGNNNNNNANNATTTNTNGNNAAAANNNNNNGNTNAAANTTTTNGNTAAANNCNNNCTCNTTTTTTTANACCAAAAG
GNCNNNAAATTCGGCAAAANNCCCTTTNNAAATTCAAAAAAANTAANACCGAANATATGGGGTTNAANNNTTTNTTCCCNAGTTTNNAAANACAGNAGTN
NCCCNTTTTTTAAAANAAANGGTGGNNNNNNNNANCNGTNNAAAGGGNGGNAAAAANCCNNTNTATTTNNGGGGGNNNNNGGNCCCCNNNNNNCNNNNNA
NNNNNNNCTACCCCNNNTANNNNNGNTTTTTTTTGGGGGGNCNCNNNNGGGNNNCCNNNAAAANNNNNNNNNAAANNNNNNNNNANNNCCCNANNANNGG
NNNACCCCNNNNNAATNTTTNNNNAANNTTTNNNNNNNNNNGNNNANNANNNNNNNNNNNNNNNNNANNNNNNNNNNNNNN
Construction:
Insert:
|
| Size: 800 bp | Source Tissue: | Stage: |
Vector:| Vector Details | Vector Map |
Name: pBluescript II SK+
Type: phagemid
5' Restriction Site: NotI
3' Restriction Site: EcoRI |  |
Usage:
|
RNA injection:
|
|
|
| |
|
Probes:
|
| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
|
T3 |
EcoRI |
HindIII or XhoI can also be used |
|
|
Whole mount in situ hybridization:
|
Expressed during stages 12 to 48 in cement gland and head ectoderm.
|
|
| |
|
Probes:
|
| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
|
T7 |
NotI |
|
|
Suppliers:
Search for clone at:
EXRC
