XB-CLONE-14575029
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Clone Name: gli1-AP450
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Gene:
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Species: Xenopus laevis
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Description: EXRC CLone Number AP-450.
Xenopus laevis zinc finger DNA binding protein Gli-1 mRNA, partial cds.
ACCESSION U57454.
1464bp partial fragment of XGliI (nucleotides 866-2329).
To generate probe for in situ and mRNA for injection.
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Sequence:
Full length sequence: pGEMT-Gli1_FullLength
[+]
gctCcggCCGcctGGCCGCgGgnttCaCCGTGgagaTGGGaTGCTCAGGGCTCAGCCcnntncgAgGGacCTGGGAATCAAAATGTTAAAGGGGAAAACC
AGCTGGATATGGAAGCAAGCTCCGCTTGCAAGGAAGATGGAAGACTTGCTGTGCCAGATATAACTTTGAAGTCTCAGCCCAGTCCTGGTGGACAGTCATC
TTGCAGCAGTGAAAGGTCCCCTTTGGGAAGCACCAACAATAATGATAGCGGTGTGGAAATGAATGCCAACACAGGAGGAAGCTTTGAGGACCTCACCAAC
CTAGATGATATTCCTTCTGTAGATTCAATGGGCACTGCAGGTGCTTCAGCCCTAAGAAAGTTGGAGAATCTTAGGATTGACAAACTGAACCAACTACGTA
AAACCCCATCCTCAGGAAAGATGGTCAAACTGCCTTCTATCCACAACAGTGGTCCTCAGGGAGATATGTCTGTTGTCTGCGGACCACTGGGTATGTCTCA
CAATCAACATGGCATAGAATTGCCAGCCAGCAGCCATGTAAATCACCTTAATGACAGGAGAAACAGCACTACAAGCACCATGAGCTCAGCCTATACTGTG
AGCCGCAGATCCTCAGTGGTGTCCCCATACCTACCCAATCAAAGAGCAGGTGATTCTGGCAACATGGTGGATTCGTATGACATTTCTACAGACCCTTCTG
GTCATTCTAATGAAGCTGTTTGTGCTAGTGGGCTTCCGGGCCTTACTCCTGCACAGCAATATAGATTAAAGGCAAAGTATGCTGCAGCAACAGGTGGTCC
TCCTCCTACCCCACTGCCCAACATGGAGAGGATGAATACAAATAACAGGATGGCATTTGCTTCTAGTGATTATCGTGGATCAGCCATCTCTTcCCTACAG
agaAGAnntAGTAGCaaTGAGTATcacaACTATGgaaCAGGTATTATTcacCCAGCTCAAGcaCCAGGTgCTGGAataannanagntAgtgACCctGCAa
gaACTGGGGga
Construction:
Insert:
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| Size: 1464 bp | Source Tissue: | Stage: |
Vector:| Vector Details | Vector Map |
Name: pGEM-T
Type: plasmid
5' Restriction Site: EcoRV
3' Restriction Site: EcoRV |  |
Usage:
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RNA injection:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| sense |
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T7 |
NotI |
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Whole mount in situ hybridization:
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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SP6 |
ApaI |
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Suppliers:
Search for clone at:
EXRC
