XB-CLONE-1570771
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Clone Name: runx3-804
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Gene:
runx3.L
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Species: Xenopus laevis
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Description: EXRC Clone Number 804.
Xenopus laevis runt-related transcription factor 3. Image EST 8462195.
To generate probe for in situ.
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Sequence:
5' EST: EB478287 
[+]
aaaanncccaatgncnntgcggaccatcgattcgaattcgtcccattcagggtccaatcgaatttaaatccaagggccacactggccaacacaaatcact
tcagcactcagacgcaaatacaagtgacaccagaattaaattctttcaccgatccccgtcagtttgaccgttcattctcagctcttccttctctcacaga
gtccaggttttccgatcctcgtatgcattatccaagtgccatgtcatctgcattctcctacactgcttccccatcaacaactggaattggtggcataagc
atgactggaatgccaactaccactcgtttccatcacacttatctaccccctccctaccctggttcatcgcaaaaccaaagcggaccattccagactaatg
catccccctaccatctctactatggtacatcatctggctcatatcaattttccatggttgcgggggagcgttctccatccagaatgctctcctcttgcac
tagtgtggcagggaataacctaatcaaccccaacttggcgaatcaaagtgatggagtagatgcagatggcagccatagcaattctcctacagccatgacc
accagtggaagaatggatgagtcagtttggagaccctattgagtgactttgtgaatacaaccacgtaattatgccccagtagaaggtcttccagncacat
atgtggtcagggatggaactatgcatatttttatgatacatgncatggggcaaccnnaatattatgaaactacaggacatctgaagaaacatacctcttn
nagatatnnactagagatctcactttacataa
Library:
Description: cDNA was primed using oligo-dT primer: 5'-GACTAGTTCTAGATCGCGAGCGGCCGCC(T)25-3' and cloned into the SmaI/NotI sites of pCS111. Size-selection >1.2kb resulted in an average insert size of 1.9 kb. This primary library was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection library.| Anatomy: spleen | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pExpress-1
Type: plasmid
5' Restriction Site: SmaI
3' Restriction Site: NotI |  |
Usage:
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Whole mount in situ hybridization:
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Expressed on Somites, eyes and BA.
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Probes:
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| Type |
Size |
Promoter |
Linearization Site |
Description |
| antisense |
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T7 |
ClaI |
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Suppliers:
Search for clone at:
EXRC
