Clone Name: uros-767
Species: Xenopus laevis
Description: EXRC Clone Number 767. Heme biosynthesis enzyme Xenopus laevis IMAGE:7206195. uroporphyrinogen III synthase (uros), mRNA. To generate probe for in situ.
Sequence:Description: RNA obtained from 6 adult male testes. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1kb resulted in an average insert size of 1.15 kb. This primary, microquantity library is normalized to Cot5 (non-normalized primary library is NICHD_XGC_Te2) and was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection library.
|Anatomy: testis||Stage: |
|Normalized: No||Subtraction: No|
|Vector Details||Vector Map||Name: pExpress-1|
5' Restriction Site: EcoRV
3' Restriction Site: NotI
Whole mount in situ hybridization:
Expressed on VBI.
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