Clone Name: gata6-HAR453
Species: Xenopus tropicalis
Description: EXRC Clone Number HAR-453. IMAGE:7722828.
Xenopus tropicalis GATA binding protein 6. Accession number CX999203.1.
To generate probe for in situ.
Sequence:Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1678 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).
|Anatomy: heart||Stage: |
|Normalized: No||Subtraction: No|
|Vector Details||Vector Map||Name: pCS107|
5' Restriction Site: EcoRI
3' Restriction Site: XhoI
Whole mount in situ hybridization:
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