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Summary Attributions
XB-CLONE-4006403

Clone Name: dkk1-HAR2 Gene: dkk1 Species: Xenopus tropicalis  
Description: EXRC Clone Number HAR-2. Full Lenght. Clone previously called: TGas131c10

Sequence:

Full length sequence: CR761605 [+]

Full length sequence: CR761605 [+]

5' EST: AL969277 [+]

3' EST: BX758076 [+]

Unigene: Str.15023


Library:

Name: XGC-gastrulaExternal Dbs: Unigene tropicalis cDNA library

Description: cDNA was oligo dT primed from 5ug of poly A+ RNA from stages 10-13 gastrulae. EcoRI-NotI cut cDNA was then ligated into pCS107 with EcoRI at the 5' end and NotI at the 3' end.

Anatomy: whole organismStage: NF stage 10.5 to NF stage 12
Normalized: NoSubtraction: No

Vector DetailsVector Map
Name: pCS107

Type: plasmid

5' Restriction Site: EcoRI

3' Restriction Site: NotI
Expression Image

Usage:

RNA injection:  
 
Probes:
Type Size Promoter Linearization Site Description
sense SP6 AscI

Whole mount in situ hybridization:  
 
Probes:
Type Size Promoter Linearization Site Description
antisense T7 EcoRI BamHI and HindIII can also be used.


Suppliers:

Search for clone at: EXRC

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Version: 4.11.0


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