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abcc8xenopus head region 

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Experiment details for abcc8

K(ATP) channel activity is required for hatching in Xenopus embryos.



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Gene Clone Species Stages Anatomy
abcc8 tropicalis NF stage 29 and 30 head region

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  Fig. 1. KATP channels are involved in hatching gland function. A,B: Immunohistochemistry using an antibody to Kir6.1 reveals the presence of KATP channels in the Y-shaped tissue of the hatching gland. C: SUR1 protein is strongly expressed in the head but is strikingly absent from the hatching gland cells, the cement gland, and the tissue between the two frontal arms of the hatching gland Y shape. D: SUR2 is present in the hatching gland. E: Control embryos hatch by stage 29. F: In contrast, embryos exposed to Nicorandil, an opener of KATP channels, remain in the vitelline membrane and are unable to hatch. G: Individual embryo showing morphology at stage 38. H: Individual embryo manually freed from the membrane shows normal development to this late stage, despite confinement to the vitelline membrane. I: Connexin30 is normally strongly expressed in the hatching gland. J: Embryos exposed to Nicorandil show a much reduced expression of Cx30 in the same tissue (white arrow). Compared with control embryos (K), the expression of the hatching enzyme is equally strong in Nicorandil-exposed embryos (L), showing that the tissue is alive and that the cells maintain hatching gland identity. Red arrows indicate expression. Green arrows indicate lack of expression

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Gene Clone Species Stages Anatomy
abcc8 tropicalis NF stage 29 and 30 head region

  abcc8 (ATP-binding cassette, sub-family C (CFTR/MRP), member 8) gene expression in a Xenopus laevis embryo, NF stage 29, as assayed by immunohistochemistry. Anterior view: dorsal up.
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