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Fig. 8. The pCar-TRDN-GFP transgene inhibits smooth muscle separation, thickening of the inter-muscular mesenchyme, and aggregation of enteric neurons. (A) The whole-mounted duodenum of an NF57 tadpole transgenic for pCar-GFP expresses GFP (green) specifically in the longitudinal (oriented left–right) and circular (oriented top–bottom) muscle fibers. Cross sections from the duodenum of (B) NF63 tadpole transgenic for pCar-GFP stained for immunoreactivity against GFP (green) and counterstained for nuclei with dapi (white); (C, D) stained with smooth muscle actin antibody; (E, F) stained with smooth muscle actin and neural beta-tubulin antibodies. (C, E) Control NF63 tadpole; (D, F) NF63 tadpole transgenic for pCar-TRDN-GFP. Circular (c) and longitudinal (l) smooth muscle fibers. Scale bar in A denotes 20 μm length; scale bar in B denotes 100 μm length; scale bar in F denotes 40 μm length. |
