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Fig. 6. The IFABP-TRDN-GFP transgene inhibits circular muscle thickening and aggregation of enteric neurons. (A) Duodenal cross sections from a wild-type NF66+ froglet; (B), from a transgenic froglet. Tissues were stained for immunoreactivity against smooth muscle actin (red) and neural beta-tubulin (green). Sections are counterstained for nuclei with dapi (white). Circular muscle fibers (c); longitudinal muscle fibers (l). Inset in B is a magnification of the region encompassed by the dashed yellow rectangle. Scale bar denotes 0.1 mm length. |
