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FIG. 4. a-SM actin expression on transversal sections of control and mutated Xenopus embryos at stage 42. In control embryos, a-SM actin
can be detected in the heart, lungs, and aorta (arrows) (A) and at the level of the presumptive intestine in a layer of cells that surrounds the
undifferentiated endodermal mass (arrowheads) (C). In mutated embryos, a-SM aetin has been detected only in the heart (B), whereas the
periphery of the undifferentiated endodermal mass, aorta, and the lung anlage (arrows) (D) remain devoid of any staining, b, brain; p, pharynx;
s, somite; n, notochord, 1, lung; h, heart; e, undifferentiated endodermal mass. Scale bar in A, B, and D, 0.3 mm; scale bar in C, 0.6 mm. |
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FIG. 5. c~-SM actin expression on transversal sections of control and mutated Xenopus embryos at stage 47. In control embryos, a-SM actin
expression covers each loop of the intestine (arrowheads); the SM cells of the lungs and the aorta are also stained (A, D); heart is negative (F).
Mutated embryos present an expression of a-SM actin in the outer layer of the incompletely differentiated intestine (arrowheads), the lumen of
which is still full of yolk (B, C, E); the SM cells of lungs and the aorta are also expressed a-SM actin (E); heart is negative (G). a, aorta; h, heart;
b, brain; n, notochord; 1, lung. Scale bar in A, B, C, F, and G, 0.3 mm; scale bar in D and E, 0.6 ram. |
