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Figure 5. Adeno-green fluorescent protein (GFP) infection of Xenopus early embryos. Embryos were injected with adeno-GFP at the indicated early stage, raised to tadpoles, and photographed for GFP fluorescence at stage 45. A: Trunk and tail muscle, injected stage 8. B: Scattered cells in liver, injected stage 8. C: Small intestine loop, injected stage 14. Bright patch on left is autofluorescence of gall bladder. D: Jaw muscles, injected stage 10.5. E: Confocal view of flattened small intestine epithelium, injected stage 14. F: Transverse confocal view of GFP (green) and smooth muscle actin (red) from small intestine, injected stage 14. G: Reverse transcriptase-polymerase chain reaction (RT-PCR) of GFP mRNA. Samples are from embryos injected with GFP RNA at two cells (top row); embryos injected with adeno-GFP as blastulae (second row); control embryos (third row); loading control (bottom row). Scale bars = 100 mu m in A-D; 50 mu m in E,F. |
