||FIGURE 3. Morpholino-mediated knockdown of WDR5 in X. laevis significantly decreased SMC-marker gene expression in vivo. In situ hybridization analyses of SM -actin (A),SM22 (E), andSM-MHC(I) mRNA expression in stage 42 X. laevis embryos injected with either control morpholinos or WDR5 morpholinos at the two-cell stage. The location of visceral muscle and the dorsal aorta are indicated with arrows, whereas arrowheads highlight embryonic regions with either reduced visceral muscle (VM) cells or dorsal aortic (DA) cells expressing SMC-marker genes. B,D, F,H, J, and L represent higher magnifications of panels A, C, E, G, I, and K, respectively.
This research was originally published in The Journal of Biological Chemistry. Gan et al “WD repeat-containing protein 5, a ubiquitously expressed histone methyltransferase adaptor protein, regulates smooth muscle cell-selective gene activation through interaction with pituitary homeobox 2.” June 17, 2011; 286 (24): 21853-21864. © The American Society for Biochemistry and Molecular Biology. Reproduced with permission.