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Experiment details for acvr2a

Dissecting BMP signaling input into the gene regulatory networks driving specification of the blood stem cell lineage.

Dissecting BMP signaling input into the gene regulatory networks driving specification of the blood stem cell lineage.

Gene Clone Species Stages Anatomy
acvr2a.L laevis NF stage 10.5 whole organism
acvr2a.L laevis NF stage 16 pre-chordal neural plate , chordal neural plate , neural plate , lateral plate mesoderm , whole organism , [+]
acvr2a.L laevis NF stage 20 pre-chordal neural plate , chordal neural plate , neural plate , lateral plate mesoderm , whole organism , [+]
acvr2a.L laevis NF stage 22 to NF stage 23 head mesoderm , lateral plate mesoderm , whole organism , dorsal lateral plate mesoderm
acvr2a.L laevis NF stage 24 to NF stage 26 head mesoderm , lateral plate mesoderm , whole organism , head mesenchyme , dorsal lateral plate mesoderm
acvr2a.L laevis NF stage 33 and 34 to NF stage 35 and 36 head mesoderm , lateral plate mesoderm , anterior dorsal lateral plate region , intersomitic region , head mesenchyme , [+]
acvr2a.L laevis NF stage 37 and 38 to NF stage 39 head region , dorsal aorta , head mesoderm , lateral plate mesoderm , anterior dorsal lateral plate region , [+]

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  Fig. S1. BMP type II receptors. Type II receptors complement type I receptors and can potentially interact with any type I receptor with ligands being the determinant of the nature of heterocomplexes. Bmpr2 is not expressed at gastrulation, but its expression is activated in the ventral mesoderm from stage 16, later extending to almost all tissues. At stages 34–39, Bmpr2 is also expressed in the DA. Acvr2a is expressed ubiquitously at all stages analyzed. Acvr2b is expressed ubiquitously at gastrulation but later display a restricted expression pattern, being expressed predominantly in neurons. Acvr2b is localized to DA at stage 39. Embryos at stages 10.5 and 34–39 are cleared to enable visualization of the staining in deeper tissues. For stage 10.5 embryos, D indicates dorsal and V indicates the ventral side. For stages 16–20, embryos are visualized from dorsal (D) and ventral (V) angles, with anterior (A) at the top, except stage 16 bmp4 staining, which is documented from an anterior–posterior (A-P) lateral view. From stage 21 onward, the pictures of embryos are taken from a lateral view, with anterior-posterior labeled where it is relevant, on the figure.