| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| acvr2a | xenopus | dorsal marginal zone [+] |
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Experiment details for acvr2a
Differential effects on Xenopus development of interference with type IIA and type IIB activin receptors.Differential effects on Xenopus development of interference with type IIA and type IIB activin receptors.
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| Fig. 2. RNAase protection analysis of XSTK9 expression. (A) Temporal expression pattern. Total RNA from two embryos was extracted at the indicated stages. (B) Spatial expression pattern at stage 8. RNA was extracted from animal caps (An), marginal zone regions (Mz). and vegetal poles (Vg). Yeast tRNA (tRNA) was used as a negative control for protection. Pigment differences were used to define dorsal (DMz), lateral (LMz) and ventral (VMz) marginal zones. To confirm these assignments, additional explants were allowed to develop to stage 10, when they were analyzed for expression of goosecoid. These results were observed in three separate experiments. Samples were co-protected with EFlor to control for amount of RNA loaded. The difference in appearance of EFlor between the XSTK9 and goosecoid protections results from the use of RNAase A with XSTK9. |