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Fig. 1. Expression of Raldh1-3 during organogenesis. In situ hybridisation of Raldh1-3, dark blue stain indicates expression. Panels A–C, H–J and O–Q are whole mount lateral views with head facing left and dorsal uppermost. Panels C’, J’ and Q’ show detail of head area. Panels D–G, K–N and R–U are paraffin sections of previously stained embryos. The blue/purple stain indicates expression and sections are counterstained with eosin (pink). (A) Raldh1 expression at stage 28. (B) Raldh1 expression at stage 30. (C) Raldh1 expression in stage 34, red arrows indicate relative positions of transverse sections in panels D to G. (C’) Detail of head of embryo shown in panel C. (D–G) Transverse sections of stage 34 embryo showing expression of Raldh1 in blue/purple. (H) Raldh2 expression at stage 28. (I) Raldh2 expression at stage 30. (J) Raldh2 expression at stage 32, red arrows indicate relative positions of transverse sections in panels K–M. (J’) Detail of head of embryo shown in panel J. (K–M) Transverse sections through stage 34 embryo showing expression of Raldh2 in dark blue. (N) Sagittal section through eye of a Raldh2 stained embryo at stage 34. (O) Raldh3 expression in stage 26 embryo. (P) Raldh3 expression in stage 30 embryo. (Q) Raldh3 expression in stage 34 embryo, red arrows indicate relative positions of transverse sections in panels R–T. (Q’) Detail of head of embryo shown in panel Q. (R–T) Transverse sections of stage 34 embryo showing expression of Raldh3 in dark blue. (U) Sagittal section of Raldh3 st. 34 embryo oriented as for whole mounts. Abbreviations: di, diencephalon; cf, choroid fissure; dr, dorsal retina; e, endoderm; ey, eye; hb, hindbrain; hy, hypophysis; le, lens; ll, lateral line precursors; lpm, lateral plate mesoderm; m/h, midbrain hindbrain boundary; no, notochord; nt, neural tube; op, olfactory placode; ov, otic vesicle; pk, pronephritic kidney; pd, pronephritic duct; re, retina; rp, roof plate; s, somite; te, telencephalon. Scale bars are 100 μM. |
