Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
aldh1a2xenopus   

Too many results?Too few results?

Experiment details for aldh1a2

Timing is everything: Reiterative Wnt, BMP and RA signaling regulate developmental competence during endoderm organogenesis.

Timing is everything: Reiterative Wnt, BMP and RA signaling regulate developmental competence during endoderm organogenesis.

Gene Clone Species Stages Anatomy
aldh1a2.L laevis NF stage 10.5 mesoderm , anterior , posterior
aldh1a2.L laevis NF stage 11.5 to NF stage 12 mesoderm , anterior , posterior
aldh1a2.L laevis NF stage 14 lateral plate mesoderm
aldh1a2.L laevis NF stage 20 mesoderm , ventro-lateral , lateral plate mesoderm
aldh1a2.L laevis NF stage 33 and 34 otic vesicle , lens , head region , lateral plate mesoderm , pronephric kidney

Display additional annotations [+]
  Supplemental Figure S2 (A). Dynamic expression of the RA-synthesizing enzymes rdh10 and raldh2 and RA-degrading enzyme cyp26a1 during early Xenopus development. Panel A shows in situ hybridization analysis of rdh10, raldh2, cyp26a1, hhex, and ventx1 at the indicated stages of Xenopus development. Abbreviations: AE, anterior endoderm; PE, posterior endoderm; AM, anterior mesoderm; PM, posterior mesoderm; vlpm, ventral lateral plate mesoderm; fg, foregut; hg, hindgut; lpm, lateral plate mesoderm. Note the robust expression of rdh10 and raldh2 in the ventral lpm at NF20 but not NF14, which correlates with the endogenous timing of RA-dependent respiratory competence of the endoderm (Supplemental Fig.S1A). Boxed flow chart in (A) is a simple schematic of where the enzymes act in the metabolism and catabolism pathway of RA biogenesis. (B). Early RA treatment of Xenopus DE is inhibitory to lung induction and promotes pancreatic/stomach and intestinal fate. Experimental diagram to test two different RA treatment periods on Xenopus gastrula DE. RT-qPCR analysis of DE explants treated either from NF10.5 to NF25 or NF15-25 with 50nM RA and then from NF25-38 with 3.5M Bio + 50ng/mL BMP4. Gene expression in each condition was normalized to the housekeeping gene ODC and then log2 fold changes in experimental gene expression were determined using the 2−δδCt method relative to the experimental gene’s ODC-normalized expression in either AE or PE cultured in isolation as indicated. Graphs display the average 2−δδCt value +/- SEM of 3 biological replicates (each biological replicate contained n=4 explants).

Gene Clone Species Stages Anatomy
aldh1a2.L laevis NF stage 11 mesoderm
aldh1a2.L laevis NF stage 20 lateral plate mesoderm , ventral mesoderm
aldh1a2.L laevis NF stage 25 head region , somite , optic vesicle , lateral plate mesoderm

  Fig. 6. Model depicting the reiterative use of Wnt, BMP, and RA signals during endoderm development. (A) In situ hybridization showing expression of the RA-synthesizing enzyme raldh2 (aldh1a2) during early Xenopus development. Abbreviations: AE, anterior endoderm; PE, posterior endoderm; lpm, lateral plate mesoderm; FG, foregut; MG, midgut; HG, hindgut; sm, somitic mesoderm; ant, anterior; post, posterior. (B) Schematic depicting the reiterative roles for Wnt, BMP and RA signals during endoderm vertebrate development.