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aldh1a2xenopus ciliary marginal zone 

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Experiment details for aldh1a2

Increased XRALDH2 activity has a posteriorizing effect on the central nervous system of Xenopus embryos.

Increased XRALDH2 activity has a posteriorizing effect on the central nervous system of Xenopus embryos.

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Gene Clone Species Stages Anatomy
aldh1a2.L laevis NF stage 33 and 34 ciliary marginal zone
aldh1a2.L laevis NF stage 35 and 36 ciliary marginal zone

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  Fig. 2. XRALDH2 is differentially expressed during Xenopus development. (A) XRALDH2 mRNA levels during Xenopus embryogenesis and in adult tissues revealed by RT-PCR. RT-PCR analysis with RNA preparations from staged embryos (embryonic stages indicated according to Nieuwkoop and Faber (1967)) and from adult tissues. Histone H4 was used as an RNA loading control. (B) Whole-mount in situ hybridization analysis using XRALDH2 antisense RNA. (1–3) Posterior view (top, dorsal; bottom, ventral); (4), dorsal view (top, anterior; bottom, posterior); (5,6), dorsal and ventral views, respectively (left, anterior; right, posterior); (7–10), anterior view; (11), lateral view; (2a), sagittal section of a stage 11 embryo; (4a,11a,11b), transverse sections (top, dorsal; bottom, ventral); (10a), the section plane is indicated by the red rectangle in panel 10. (C) Double staining whole-mount in situ hybridization of XRALDH2 (red) and XCYP26 (blue). (1–3), posterior view (top, dorsal; bottom, ventral); (4), dorsal view (top, posterior; bottom, anterior); (5), anterior view; (6), lateral view; (2a), sagittal section; (6a), higher magnification of the head region of 6; (6b, 6c), frontal section (top, dorsal; bottom, ventral); (6d), horizontal section (top, anterior; bottom, posterior). Red dashed lines and rectangles in (B) and (C) indicate the locations for vibratome sections. Nieuwkoop–Faber stages of embryogenesis are indicated in the lower right corner of each picture ( Nieuwkoop and Faber, 1967). (B,C) ar, Archenteron; cg, cement gland; dc, diencephalon; dz, deep zone of involuting marginal zone; ec, ectoderm; el, epithelial layer of involuting marginal zone; ele, epithelial layer of ectoderm; ls, lens; m, mesoderm; mc, mesencephalon; md, developing mesonephric duct; mg, midgut; m/h, midbrain/hindbrain boundary expression of XCYP26; no, notochord; oe, oral epithelia; op, olfactory placode; pc, prosencephalon; pe, pigment epithelium of retina; r3, rhombomere 3 domain of XCYP26; rc, rhombencephalon; re, retina; sc, spinal cord; sh, stomodeal–hypophyseal anlage; sl, sensorial layer of ectoderm; zii, zone of internal involution.
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