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Figure S2: Retinal lamination is affected upon Sipa1l3 and Epha4 loss of function in Xenopus.
Sipa1l3 as well as Epha4 depletion results in disorganized retinal layers in the eye including rosette-like structures formed by displaced photoreceptor cells from the outer layer (black arrowheads) to the inner layers (red arrowheads) of the retina. Following marker genes were used to stain for retinal cell types at stage 42: arr3 and rho to determine photoreceptor cells; pax6 to detect amacrine and ganglion cells; vsx1 to visualize bipolar cells and pou4f1 to stain for ganglion cells. B qPCR experiments using cDNA of Sipa1l3 or Epha4 depleted stage 42 eyes revealed a down-regulation of rho, whereas the other analyzed marker genes were slightly up-regulated. CoMO injected embryos were used as control. Gapdh was used for normalization. n, number of independent experiments; ng, nanogram. Error bars indicate standard error of the means (s.e.m.); * p0.05; ** p0.01. P-values were calculated by a nonparametric, one-tailed Mann-Whitney rank sum test. |
