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FIG. 2. Comparison of X-Notch-1 and achaete-scute-like gene expression patterns in stage 40 Xenopus retina. (A) Comparison of X-Notch-1 and Xash1 expression patterns. Double in situ hybridizations were performed as in Fig. 1 with Xash1 in deep purple and X-Notch-1 in red. (A) Xash1 expression is detected in the CMZ except in cells in the most peripheral region. (B) X-Notch-1 labeling is also detected in the CMZ except in cells in the most peripheral region. C and D show a higher magnification of the CMZ from the sections depicted in A and B, respectively. (E) Double labeling demonstrates that Xash1 and X-Notch-1 expressions overlap in the CMZ. Scale bar in A and B, 100 um; scale bar in C, D, and E, 30 um. (F) Comparison of X-Notch-1 and Xash3 expression patterns. Double in situ hybridizations were performed as in Fig. 1 with Xash3 in deep purple and X-Notch-1 in red. (F) Xash3 expression is detected in the CMZ except in cells in the most peripheral region. (G) X-Notch-1 labeling is also detected in the CMZ except in cells in the most peripheral region. H and I show a higher magnification of the CMZ from the sections depicted in F and G, respectively. (J) Double labeling demonstrates that Xash3 and X-Notch-1 expressions do not exactly overlap in the CMZ, since some X-Notch-1 positive cells in the most central region of the CMZ do not express Xash3 (arrow). Scale bar in F and G, 100 um; scale bar in H, I, and J, 30 um. |
