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FIG. 1. Inducible Xash1 and XNgnr1 function. (A) (i–iii) Xash1 and XNgnr1 expression and function in Xenopus embryos. Xash1 (i),
XNgnr1 (ii), and N-tubulin (iii) expression in Xenopus embryos at stage 15. (i) Asterisks indicate three bilaterally symmetric domains of
Xash1 expression. Arrowhead indicates medial expression domain. (ii and iii) Asterisks indicate domains of primary neurogenesis in the
embryo. (iv–vi) Hormone-inducible Xash1 and XNgnr1 function in the embryo. Embryos injected with GRXash1 (iv and v) or GRXNgnr1
(vi) and incubated either in the absence (iv) or presence (v and vi) of Dex to stage 15/16. N-tubulin expression marks domains of ectopic
neurogenesis (dark blue/purple staining) and -gal (blue) expression serves as a lineage marker. (B) PCR analysis of GRXash1 and GRXNgnr1
function in Xenopus animal caps. Sequence-specific primers were used to assay N-tubulin (N-tub) and Neurofilament-M (NF-M) expression
in GRXash1- and GRXNgnr1-injected animal cap samples incubated for 20 h with (D) or without ( ) Dex. Embryo (E) and uninjected animal
caps exposed to Dex for 20 h (C) were used as controls in these experiments. Expression of muscle specific actin (MSA) was used to control
for mesodermal contamination, and expression of EF1 served as an internal quantitation control. |
