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FIG. 1. Onset of BDNF expression in the developing optic tectum. In situ hybridization with a digoxygenin-labeled antisense BDNF probe
shows specific alkaline phosphatase reaction signals over the cytoplasm of midbrain cells of stage 40 and 45 tadpoles. (A) Transverse cryostat
section at the level of the rostral midbrain of a stage 40 tadpole shows weak digoxygenin-positive cell bodies in dorsal and ventral midbrain
cells. (B) An adjacent section to that in A, hybridized with a sense control BDNF probe shows no signal. (C) Sagittal section of a stage 40 tadpole
reveals that the highest BDNF-expressing cells are localized to the ventral part of the rostral midbrain, presumably in the developing ventral
mesencephalic nuclei (vmn). (D) Hybridization of a stage 45 tadpole with the antisense BDNF probe shows specific hybridization in ventral
mesencephalic nuclei (vmn) as well as in dorsolateral cells laying in close proximity to the tectal neuropil (np). (E) Hybridization of a stage 45
transverse section at the level of the rostral midbrain with a sense control BDNF probe shows no signal. D, diencephalon, M, mesencephalon;
me, melanocyte; R, rhombencephalon; T, telencephalon. Dorsal is up, ventral is down. Scale bar, 50 mm. |
