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FIG. 4. In situ hybridization for BDNF and trkB shows the colocalization of these two messages to particular retinal areas. Fifteenmicrometer
alternate cryostat sections of stage 45 Xenopus retina were hybridized with digoxygenin-labeled antisense Xenopus BDNF
(A, C, E) or trkB probes (B, D, F). Specific cytoplasmic hybridization signals were localized to the ganglion cell layer (gcl) after hybridization
with the BDNF probe (A, C, E), whereas specific hybridization signals were localized to both the ganglion cell layer (gcl) and innermost
part of the inner nuclear layer (inl) after hybridization with the trkB probe (B, D, F). High-magnification photomicrographs of adjacent
retinal sections hybridized with these two probes revealed that both BDNF (E) and trkB (F) digoxygenin-positive cell bodies colocalize to
the same area in the ganglion cell layer. Arrowheads and asterisks point to two groups of cells, which are positive for the BDNF (E) and
trkB (F) probes, respectively. The retinal areas outlined by the brackets in C and D are shown as high-magnification photomicrographs
in E and F, respectively. Scale bar for E and F is 20 mm. |
