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Fig. I. Expression of BDNF mRNA in the vestibular and auditory endorgans. Sections a--e were hybridized with the BDNF antisense riboprobe.
Section f was prepared with the BDNF sense riboprobe, a,c,d: Darkfield photomicrographs show strong BDNF hybridization in the (a)
macular sacculi, (c) amphibian papilla, and (d) amputlary cristae. Scale bar= 35 gm. b,e: Brightfield photomicrographs demonstrate high levels
of BDNF transcripts in the (b) macular sacculi and (e) ampullary cristae. Scale bar = 17 ~tm. f: Darkfield photomicrograph demonstrates sense
riboprobe control in section adjacent to c. Note the lack of specific hybridization. Scale bar = 35 gm. |
